{"signature": "0x2e3178da86e4d1bb98d551f6b8b48e600b71f3699865275b01b7786c2e6855f859a9b1e860549781e2d8a29775adb71795ab06ffcaf7b2f6ad2d82eeec594b261c", "application": {"round": "0x0C0412DDB846096Ea1e37de717921EBF4fEF9A39", "answers": [{"type": "email", "answer": "r@overlake.bio", "hidden": true, "question": "Email Address", "questionId": 0}, {"type": "paragraph", "answer": "**27,500 USD**\nVitaDAO, Longevity Hackathon, Longevity Prize, PrivateAI, ResearchHub, GG20/21, FunDeSci, personal donations.", "hidden": false, "question": "Funding Sources and Total prior funding for project in USD", "questionId": 1}, {"type": "number", "answer": "1", "hidden": false, "question": "Team Size", "questionId": 2}, {"type": "paragraph", "answer": "[Rakhan Aimbetov](https://x.com/r_aimbetov)", "hidden": false, "question": "Profiles or socials of other main team members publicly associated with project", "questionId": 3}, {"type": "short-answer", "hidden": true, "question": "Telegram username", "questionId": 4}, {"type": "short-answer", "answer": "40", "hidden": false, "question": "How old is the project? (Months)", "questionId": 5}, {"type": "paragraph", "answer": "## **Problem**  \nWhile most efforts in the longevity biotechnology industry are directed toward intracellular processes, **overlake.bio** explores the cellular environment, represented by the **extracellular matrix** (ECM). Operating at the intersection of molecular biology, mechanobiology, and biochemistry, the project offers a comprehensive framework for targeting hitherto irreparable forms of age-related damage.\n\n## **Solution**  \n*De novo* engineering of enzymes with enhanced functionalities and/or custom target specificities opens broad possibilities for achieving cellular resilience, damage repair, and carrying out novel reactions, working towards optimal human health and longevity. The project aims to ameliorate the mechanosensory burden of the aged ECM through computational biology and reaction-conditioned enzyme engineering to promote youthful ECM remodeling. With its scope outside the cell-centric view on aging, the project addresses a critical conceptual and technological gap within modern longevity research, aiming to extend the healthy human lifespan by leveraging the body’s intrinsic ability to restore itself, engineering the ECM turnover.", "hidden": false, "question": "Describe your project in a few paragraphs (ideally including a problem statement, solution, roadmap, and timeline for implementation)", "questionId": 6}, {"type": "paragraph", "answer": "**overlake.bio** is an example of a fully decentralized hard science initiative, funded exclusively through web3 mechanisms and independently executed. We work toward establishing DeSci as a valid professional career path for scientists, a viable alternative to academia and industry.", "hidden": false, "question": "How does your project support the mission or goals of DeSci?", "questionId": 7}, {"type": "paragraph", "answer": "The funds will be used to support **overlake.bio**’s publication activities planned for 2025:  \n- Revision and submission to a peer-reviewed journal of the [preprint](https://dx.doi.org/10.2139/ssrn.4566939) describing our findings on the effects of ribosomal glycation on mRNA decoding accuracy.  \n- Finalization and submission to a peer-reviewed journal of a collaborative [review paper](https://doi.org/10.55277/researchhub.rb3ck05l) on popular longevity biomarkers.  \n- Finalization and publication of a preprint entitled (tentatively): **Production of the recombinant human glyoxalase DJ-1/PARK7 in methylotrophic yeast *Pichia pastoris***  \n- Finalization and publication of a preprint entitled (tentatively): **Bacterial expression and purification of the recombinant human histone H3.1 for probing electrophilic adduction *in vitro***", "hidden": false, "question": "Describe how funds from the grants program will be used for your project.", "questionId": 8}, {"type": "paragraph", "hidden": false, "question": "If you participated in past grant rounds (i.e. DeSci GR15/ Beta/GG 20DeSci round/GG21 DeSci round) using a different project payout wallet address, please share it here.", "questionId": 9}, {"type": "paragraph", "answer": "Please see our updates on [Karma GAP](https://gap.karmahq.xyz/project/overlake--bio).", "hidden": false, "question": "If this project participated in past DeSci grant rounds, please share any new updates or milestones since the last round.", "questionId": 10}, {"type": "paragraph", "hidden": true, "question": "Is there any other information you would like to share about your project, previous work, other project affiliations, or potential conflicts of interest? (or anything not covered elsewhere in this application that you feel is relevant to share)", "questionId": 11}, {"type": "dropdown", "answer": "Yes", "hidden": false, "question": "Does 50% of your team reflect the diversity of the community your project serves with a particular emphasis on including women, Black, Indigenous, and People of Color (BIPoC), individuals with disabilities, and members of the LGBTQ+ community?", "questionId": 12}], "project": {"id": "0x1a1c47a3bad42e4f700bfb6ccd00cef0d142e581e824a82f958c2fbf22bac856", "title": "overlake · bio", "logoImg": "bafkreibuepyfa5zwradelsp2xxig2fgz6s6p6n7co5nttd2zriy5yk2bpe", "metaPtr": {"pointer": "bafkreihryg6w77yf5b5c6ikmlgi6jsmeqpbcmdrptte4ft4cfged4llvpq", "protocol": "undefined"}, "website": "https://overlake.bio", "bannerImg": "bafkreig4mlbdkxyysiyxk7dp76de5dvjedbnj4zxhitx662nsgijlnu33i", "createdAt": 1743351619742, "credentials": {"twitter": {"type": ["VerifiableCredential"], "proof": {"type": "EthereumEip712Signature2021", "created": "2025-03-30T16:19:23.851Z", "@context": "https://w3id.org/security/suites/eip712sig-2021/v1", "proofValue": "0x51c9016952cee69e08b440c0d8159651faac0277753f9417419118e95212cae95391e4e2bafbcac37be7c34a4a679b57c9831738709a8c7b94bab02b121e39ec1c", "eip712Domain": {"types": {"Proof": [{"name": "@context", "type": "string"}, {"name": "created", "type": "string"}, {"name": "proofPurpose", "type": "string"}, {"name": "type", "type": "string"}, {"name": "verificationMethod", "type": "string"}], "@context": [{"name": "nullifiers", "type": "NullifiersContext"}, {"name": "provider", "type": "string"}], "Document": [{"name": "@context", "type": "string[]"}, {"name": "credentialSubject", "type": "CredentialSubject"}, {"name": "expirationDate", "type": "string"}, {"name": "issuanceDate", "type": "string"}, {"name": "issuer", "type": "string"}, {"name": "proof", "type": "Proof"}, {"name": "type", "type": "string[]"}], "EIP712Domain": [{"name": "name", "type": "string"}], "CredentialSubject": [{"name": "@context", "type": "@context"}, {"name": "nullifiers", "type": "string[]"}, {"name": "id", "type": "string"}, {"name": "provider", "type": "string"}], "NullifiersContext": [{"name": "@container", "type": "string"}, {"name": "@type", "type": "string"}]}, "domain": {"name": "VerifiableCredential"}, "primaryType": "Document"}, "proofPurpose": "assertionMethod", "verificationMethod": "did:ethr:0xd6f8d6ca86aa01e551a311d670a0d1bd8577e5fb#controller"}, "issuer": "did:ethr:0xd6f8d6ca86aa01e551a311d670a0d1bd8577e5fb", "@context": ["https://www.w3.org/2018/credentials/v1", "https://w3id.org/vc/status-list/2021/v1"], "issuanceDate": "2025-03-30T16:19:23.850Z", "expirationDate": "2025-06-28T16:19:23.850Z", "credentialSubject": {"id": "did:pkh:eip155:1:0x59041d70deAEfe849A48E77e0b273DdD072eA9e4", "@context": {"provider": "https://schema.org/Text", "nullifiers": {"@type": "https://schema.org/Text", "@container": "@list"}}, "provider": "ClearTextTwitter#overlake_bio", "nullifiers": ["v0.0.0:KltcCQiei6lgpYRgzsi01s7JYkvuL85lYmwFNmSFu2s="]}}}, "description": "**overlake.bio** is an end-to-end decentralized science (**DeSci**) framework comprising a collection of [projects](https://overlake.notion.site/all-projects-0b1edcacb77e4c93a1b8ad8df47c161d) aimed at promoting human longevity.\n\nThe framework comprises four projects:\n\n**1| [Enzyme engineering for crosslinked collagen turnover](https://overlake.notion.site/Enzyme-engineering-for-crosslinked-collagen-turnover-17f3fc8f169f80079fbfe1fdaed87d0a)**  \nTo design matrix metalloproteinases for extracellular matrix rejuvenation.\n\n**2| [Extracellular matrix aging atlas](https://overlake.notion.site/Extracellular-matrix-aging-atlas-10e0f2ecd2f545afad43fd971cce5be7)**  \nTo assemble a knowledgebase of time-resolved matrisome signatures extracted from public proteomic datasets.\n\n**3| [Advanced glycation end product (AGE)-degrading enzymes](https://overlake.notion.site/Advanced-glycation-end-product-AGE-degrading-enzymes-ce317852187b4639b5e5debe42b62290)**  \nTo engineer biologics to alleviate the glycation load.\n\n**4| [Glycation of ribosomes as a driver of proteostasis loss](https://overlake.notion.site/Glycation-of-ribosomes-as-a-driver-of-proteostasis-loss-d8971d87778846349d8f4fc29a91a30b)**  \nTo produce novel insights into translation fidelity in aging. \\[[preprint](https://dx.doi.org/10.2139/ssrn.4566939)\\]", "lastUpdated": 0, "projectTwitter": "overlake_bio"}, "recipient": "0x59041d70deAEfe849A48E77e0b273DdD072eA9e4"}}